FLT3 (fms-related tyrosine kinase 3) gene frequently mutated in acute myeloid leukemia (AML) is associated with disease pathogenesis. Interna tandem duplications in juxtamembran region of cytoplasmic domain of FLT3 gene is observed in 17-34% of leukemic blast cells of AML patients. Onkotest K3030-20 kit is specifically designed to detect those duplications and also can be used detect point mutations (D835/I836) observed in 7% of AML patients.